In recent years, dental tissue–derived stem cells have emerged as a versatile and accessible source for regenerative medicine, with potential applications ranging from minimally invasive therapies to advanced tissue engineering. This hands-on workshop invites students to explore the entire continuum—from isolation and characterization to expansion, subculture, and evaluation of material safety and compatibility when integrating stem cells into regenerative strategies. Through carefully guided wet-lab activities, participants will gain practical competence in sterile technique, cell culture fundamentals, and the assessment of materials and products used with dental stem cells, equipping them with skills directly transferable to research and clinical contexts. Participants will engage in a structured, student-centered learning experience that emphasizes critical thinking, safety, and reproducibility. By integrating demonstrations with active practice, the course helps attendees translate theoretical concepts into laboratory proficiency, fostering confidence in handling dental-derived stem cells and in evaluating regenerative medicine approaches for safety and efficacy. Whether shaping a future career in regenerative dentistry or biomedical research, this workshop provides a solid foundation for responsible innovation in stem cell science.
The Speakers
1
Shereen Nader
Associate Professor
Sponsored by:
Course Topics
- By the end of this workshop, participants will be able to:
- 1. Perform sterile technique basics and set up a clean, safe laboratory workstation for dental tissue–derived stem cell work.
- 2. Explain the rationale for using dental tissues as a stem cell source and describe key cell surface markers and basic characterization approaches.
- 3. Isolate dental tissue–derived stem cells from chosen sources using approved, stepwise methods, minimize contamination risk, and select suitable isolation strategies for downstream culture.
- 4. Culture and subculture (expanding) dental stem cells with proper culture medium handling, passaging, and contamination prevention.
- 5. Apply basic cryopreservation principles and manage simple post-thaw viability checks and diagnose potential cryoinjury in lab assays.
- 6. Design a small, concept-driven in vitro experiment or workflow that demonstrates safe integration of stem cells with regenerative material
- 7. Interpret basic results from lab experiments, discuss potential implications for research, and communicate findings clearly in a scientific context..
- 8. Demonstrate adherence to safety, ethical considerations, and regulatory guidelines relevant to dental stem cell work in the laboratory.